Quantification of Angora goat beta chain haemoglobin variants using the Sebia Capillarys 2 Flex-Piercing system

Authors

DOI:

https://doi.org/10.36303/JSAVA.683

Keywords:

capillary electrophoresis, haemoglobin switching, neonatal

Abstract

This study aimed to evaluate the utility of the Sebia Capillarys 2 Flex-Piercing (C2FP) system for the quantification of the major caprine beta chain haemoglobin (Hb) variants, i.e. foetal Hb, neonatal Hb, and adult Hb. Blood samples were collected from six Angora kids at one, 11, and 20 weeks after birth, and analysed using the C2FP instrument and Capillarys Hemoglobin(E) kit. Electropherograms displayed three major peaks which showed consistent changes in relative magnitude as kids aged. At one week old, a peak at position ~75 was identified as foetal Hb, while in older animals, peaks at ~150 and ~170 were identified as neonatal Hb and adult Hb, respectively. In conclusion, this study has confirmed the utility of the Sebia C2FP system for the simple, rapid, and standardised quantification of the major beta chain Hb variants of Angora goats.

Author Biographies

  • SDC Parsons, Stellenbosch University

    South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University and Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, South Africa

  • A Vermeulen, Stellenbosch University

    South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa

  • M Hobson, Mohair SA

    Mohair SA, Camdeboo Veterinary Clinic, South Africa

  • EH Hooijberg, University of Pretoria

    Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, South Africa

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Published

2025-08-27

Issue

Vol. 96 No. 1 (2025)

Section

Short Communications

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Copyright (c) 2025 Author/s

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.