Search this journal:     Advanced search
Articles

Collection of preputial material by scraping and aspiration for the diagnosis of Tritrichomonas foetus in bulls

P.C. Irons, M.M. Henton, H.J. Bertschinger

Journal of the South African Veterinary Association; Vol 73, No 2 (2002), 66-69. doi: 10.4102/jsava.v73i2.558

Submitted: 06 July 2002
Published:  06 July 2002

Abstract

Two trials were carried out to assess the diagnostic sensitivity and practicability of preputial scraping as a method of collecting preputial material from bulls infected with Tritrichomonas foetus. In the 1st trial, preputial material was collected by simultaneous scraping and aspiration from 3 infected and 1 uninfected bull 10 times over a 5-week period. In the 2nd trial, samples from 5 infected bulls were collected by both sheath washing and scraping on 6 occasions, while 8 uninfected animals were sampled 3 times. Samples were cultured using a modified Trichomonas culture medium (Oxoid). In the first trial, 29 of 30 samples from infected bulls were found to be positive. In the second trial, 83 % of samples collected by both methods tested positive. In neither trial were any samples from the control bulls found to be positive. Scraping was found to be quick and safe, and offered advantages over preputial washing in that urine contamination was easily avoided, samples were smaller and more concentrated and contamination was reduced. It may, however, be subject to greater operator variability than sheath washing. It is concluded that preputial scraping is as effective as washing and represents a suitable alternative for the collection of material for direct examination and culture of Tritrichomonas foetus.

Full Text:  |  PDF (58KB)

Author affiliations

P.C. Irons,
M.M. Henton,
H.J. Bertschinger,

Keywords

Bull; Diagnosis; Preputial Scraping; Preputial Wash; Tritrichomonas Foetus; Venereal Disease

Metrics

Total abstract views: 1359
Total article views: 2282  

Cited-By

1. High prevalence of Tritrichomonas foetus infection in Asturiana de la Montaña beef cattle kept in extensive conditions in Northern Spain
Jesús A. Mendoza-Ibarra, Susana Pedraza-Díaz, Francisco J. García-Peña, Silvia Rojo-Montejo, Jose A. Ruiz-Santa-Quiteria, Elena San Miguel-Ibáñez, Vanesa Navarro-Lozano, Luis M. Ortega-Mora, Koldo Osoro, Esther Collantes-Fernandez
The Veterinary Journal  vol: 193  issue: 1  first page: 146  year: 2012  
doi: 10.1016/j.tvjl.2011.09.020

Comments on this article

Before posting your comment, please read our policy.
Post a Comment (Login required)


ISSN: 1019-9128 (print) | ISSN: 2224-9435 (online)

Connect on: Facebook, Twitter, Google+, LinkedIn and YouTube

Subscribe to our newsletter

All articles published in this journal are licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license, unless otherwise stated.

Website design & content: ©2017 AOSIS (Pty) Ltd. All rights reserved. No unauthorised duplication allowed.

AOSIS Publishing | Empowering Africa through access to knowledge
Postnet Suite #110, Private Bag X19, Durbanville, South Africa, 7551
Tel: 086 1000 381
Tel: +27 21 975 2602
Fax: 086 5004 974

publishing(AT)aosis.co.za replace (AT) with @

Please read the privacy statement.