Blood acid–base, haematological and haemostatic effects of hydroxyethyl starch (130/0.4) compared to succinylated gelatin colloid infusions in normovolaemic dogs

Authors

  • Roxanne K Buck University of Pretoria
  • Lynette Bester University of Pretoria
  • Keagan J Boustead University of Pretoria
  • Abdur R Kadwa University of Pretoria
  • Gareth E Zeiler University of Pretoria

Abstract

Synthetic colloids are commonly administered to dogs to treat absolute or relative hypovolaemia. Voluven® (tetrastarch 130/0.4) and Gelofusine® (succinylated gelatin) are available to veterinarians in South Africa. In humans, use of these products has caused acid– base derangements, changes in haematology and impaired haemostasis. We aimed to investigate these effects in healthy normovolaemic dogs. Eight healthy adult beagle dogs underwent a cross-over study, receiving Voluven® or Gelofusine® (10 mL/kg/h for 120 min) once each with a 14-day washout between treatments. Dogs were premedicated with dexmedetomidine (10 µg/kg intramuscularly). Anaesthesia was induced with propofol and the dogs were maintained with isoflurane-in-oxygen. The anaesthetised dogs were connected to a multi-parameter monitor to monitor physiological parameters throughout. Catheters
placed in a jugular vein and dorsal metatarsal artery allowed sampling of venous and arterial blood. Blood was collected immediately prior to commencement of colloid infusion, after 60 min infusion and at the end of infusion (120 min) to allow for arterial blood gas analysis, haematology and coagulation testing (activated partial thromboplastin time [aPTT], prothrombin time [PT] and thromboelastography [TEG]). There was no effect, between treatments or over time, on blood pH. The haemoglobin concentration, erythrocyte count and haematocrit decreased significantly over time (all p < 0.01), with no differences between
treatments, and remained within normal clinical ranges. There were no differences between treatments or over time for the TEG, aPTT and PT tests of haemostasis. At the dose studied, Voluven® and Gelofusine® had comparably negligible effects on blood acid–base balance and coagulation in normovolaemic dogs. Keywords: acid–base balance; coagulation; Gelofusine; synthetic colloids; Voluven; full blood count (FBC); complete blood count (CBC).

Author Biographies

  • Roxanne K Buck, University of Pretoria

    Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria,
    Onderstepoort, South Africa

  • Lynette Bester, University of Pretoria

    Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria,
    Onderstepoort, South Africa and Companion Animal Health Centre, School of Animal and Veterinary Science, University of Adelaide, Adelaide, Australia

  • Keagan J Boustead, University of Pretoria

    Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria,
    Onderstepoort, South Africa and Section of Anaesthesia and Critical Care, Valley Farm Animal Hospital, Pretoria, South Africa

  • Abdur R Kadwa, University of Pretoria

    Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria,
    Onderstepoort, South Africa

  • Gareth E Zeiler, University of Pretoria

    Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria,
    Onderstepoort, South Africa and Section of Anaesthesia and Critical Care, Valley Farm Animal Hospital, Pretoria, South Africa

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Published

2021-12-09

Issue

Section

Original Research